Journal of Biotechnology
Volume 1, No. 1, 2022
Pages 56-65
DOI: 10.36108/jbt/2202.10.0150
Dilute Sulphuric Acid Pre-treatment for Efficient Production of Bioethanol from Sugarcane Bagasse using Saccharomyces cerevisiae
ABUBAKAR, Abdurrahman 1, PETER Richard1, AHMAD, Ibrahim Galadima 1, ADAMU, Ubaida Muhammad 2, MUHAMMED, Adamu 3
1Department of Biological Sciences, Faculty of Science, Federal University of Kashere, Gombe, Gombe State, P.M.B. 0182, Nigeria
2Institute of Life Sciences and Biomedical, Far Eastern Federal University, Vladivostok, Primorsky Krai. 690091. Russia.
3Biology Education Department, School of Science, Adamawa State College of Education Hong, Adamawa state. Nigeria
*Corresponding author: Email:abdulrahmanabubakar880@gmail.com Orcid: https://orcid.org/0000-0002-0061-6839
Abstract
In the present study, the effect of dilute acid hydrolysis during the pre-treatment of sugarcane bagasse (SCB) was conducted using sulphuric acid of various concentrations. A high concentration of reducing sugar of 270.90 g/L was achieved by soaking bagasse in 0.5M of H2SO4 at a constant temperature of 120 oC. After the pre-treatment (saccharification), the reducing sugars obtained at the different concentrations were fermented by Saccharomyces cerevisiae mono-culture modes. The results indicated that, the pre-treated SCB treated with 0.5M H2SO4 produced the highest yield of ethanol which was 8.884 g/L with a productivity of 0.134 g/L/h. However, relatively low ethanol yield was found when 0.2M H2SO4 was used which yielded 8.2185 g/L. In any case, acid hydrolysis proves to be a better method of hydrolysis and an increase in the concentration during hydrolysis contributes to higher sugar yield and subsequently higher ethanol yields.
Keywords: ABE fermentation; bioethanol; Saccharomyces cerevisiae; sugarcane bagasse; sulphuric acid